Journal:

Article Title: Diverse and Specific Gene Expression Responses to Stresses in Cultured Human Cells D⃞

doi: 10.1091/mbc.E03-11-0799

Figure Lengend Snippet: Conditions and cell lines used for microarray experiments

Article Snippet: Cell Culture Conditions Culture conditions were as follows: HeLa S3 cervical carcinoma cells ( Puck et al ., 1956 ) were grown in DMEM supplemented with 10% fetal bovine serum, 100 U of penicillin-streptomycin, and 1× nonessential amino acids at 37°C with 5% CO 2 ; normal human diploid lung fibroblasts (American Type Culture Collection, Manassas, VA) were grown under the same conditions as HeLa cells but with 20% serum; and K-562 cells ( Lozzio and Lozzio, 1975 ) were grown in spinner flasks at 37°C in Iscove's modified Dulbecco's medium supplemented with 10% fetal bovine serum and 100 U/ml penicillin-streptomycin.

Techniques: Microarray

Journal:

Article Title: Diverse and Specific Gene Expression Responses to Stresses in Cultured Human Cells D⃞

doi: 10.1091/mbc.E03-11-0799

Figure Lengend Snippet: Cell line-dependent gene expression patterns. Data for cell line specific clusters are shown with the same color scheme as in Figure 1. Clusters of genes whose expression was repressed by stress in fibroblasts (A), induced by stress in HeLa cells (B), or induced by redox stress in fibroblasts (C) were produced using the two-step clustering method described in MATERIALS AND METHODS. (D) We extracted and clustered the data for 1134 clones found to be cell cycle regulated by (Whitfield et al., 2002 ). The genes shown are the core of the resulting cluster and contain the most strongly cell cycle-regulated genes. (E) Data for all of the genes encoding ribosomal proteins present on the arrays were extracted and the genes were ordered by hierarchical clustering.

Article Snippet: Cell Culture Conditions Culture conditions were as follows: HeLa S3 cervical carcinoma cells ( Puck et al ., 1956 ) were grown in DMEM supplemented with 10% fetal bovine serum, 100 U of penicillin-streptomycin, and 1× nonessential amino acids at 37°C with 5% CO 2 ; normal human diploid lung fibroblasts (American Type Culture Collection, Manassas, VA) were grown under the same conditions as HeLa cells but with 20% serum; and K-562 cells ( Lozzio and Lozzio, 1975 ) were grown in spinner flasks at 37°C in Iscove's modified Dulbecco's medium supplemented with 10% fetal bovine serum and 100 U/ml penicillin-streptomycin.

Techniques: Gene Expression, Expressing, Produced, Clone Assay

Journal: Oncotarget

Article Title: Inhibition of primary breast tumor growth and metastasis using a neuropilin-1 transmembrane domain interfering peptide

doi: 10.18632/oncotarget.10101

Figure Lengend Snippet: A. Representative western blots showing the induction of AKT phosphorylation upon treatments with 200ng/ml Sema3A and with or without MTP-NRP1. Akt and p-AKT expression levels were normalized with GAPDH. B. Quantitative analysis of 3 independent experiments showing the relative expression of p-AKT/AKT in the different experimental conditions. (*= p<0.05, ns: not significant; Mann Whitney test).

Article Snippet: MDA-MB-231 cells were seeded on 6-well plates overnight, and then treated with 200ng/ml Sema3A (R&D 1250-S3) for 30 minutes prior to addition of 10 −6 M of MTP-NRP1 for one additional hour.

Techniques: Western Blot, Expressing, MANN-WHITNEY